The Effect of Some Plant Growth Regulators and Their Combination with Methyl Jasmonate on Anthocyanin Formation in Roots of Kalanchoe Blossfeldiana

In this study, we investigated the effect of plant growth regulators (PGRs) – auxins, gibberellin, cytokinin, abscisic acid, brassinosteroid, ethylene and their interaction with methyl jasmonate (JA-Me) applied to roots of the whole plants Kalanchoe blossfeldiana on the accumulation of anthocyanins in roots. The highest stimulation of anthocyanins synthesis was stated with application of JA-Me alone. In response to treatments with the other tested PGRs, the content of anthocyanins in roots of a whole plant was different depending on the concentration of the PGR when being applied alone or together with JA-Me. Auxin, indole-3-acetic acid (IAA) at a concentration of 50 mg·L, indole-3-butyric acid (IBA) at 5 mg·L and abscisic acid (ABA) at 10 mg·L induced anthocyanin accumulation with approximately 60-115% compared to the control while 24-epibrassinolid (epiBL), gibberellic acid (GA3) and 6-benzylaminopurine (BAP) had no effect on the anthocyanin accumulation. The simultaneous administration of the PGRs with JA-Me usually resulted in the accumulation of anthocyanins in roots in a manner similar to that caused by JA-Me. PGRs applied to isolated roots did not stimulate anthocyanin accumulation, except for the combination of JA-Me with 50 mg·L IAA. The results indicate that in K. blossfeldiana, the aboveground parts of the plant play an important role in the biosynthesis of anthocyanins in roots.